7/3/2023 0 Comments The Pen, Inc by J.O. Simon![]() ![]() ![]() Approach the tissue surface with the AFM cantilever by switching on the laser and clicking on the approach key. Then specify the file name and directory for saving the measurement files by going to the setup tab and clicking on saving settings. In the atomic force microscopy or AFM software, go to the setup tab and mark a tick on autosave to automatically save all files. Cover the sample with PBS, then load the sample onto the AFM stage, and cover it with the AFM head. Fix the sections with ice cold 4%paraformaldehyde in PBS for 10 minutes at four degrees Celsius, followed by washing five times with PBS.Īfter the last wash, wipe the excess PBS around the liver section using tissue, and mark a boundary of approximately two by four centimeters around the liver section with a hydrophobic marker pen. ![]() To begin, remove the frozen liver sections from minus 80 degrees Celsius, and thaw them at room temperature for two minutes. Our protocols can provide great insight into the development of liver fibrosis, and it can also be adopted for other soft tissues such as lungs after certain optimizations. The main advantage of the technique lies in the use of polarizing microscopy to locate collagen rich areas in the liver sections. It can thus be used to study the processes of fibrogenesis in the greater details. ![]() Our protocol provides a distinction between collagen rich and collagen lacking areas in the liver sections. ![]()
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